Medium for Screening Leuconostoc oenos Strains Defective in Malolactic Fermentation

Author:

Cavin J. F.1,Prevost H.1,Lin J.1,Schmitt P.1,Divies C.1

Affiliation:

1. ENS.BANA, Laboratoire de Microbiologie, Université de Bourgogne, Campus Universitaire Montmuzard, 21000 Dijon, France

Abstract

A new sensitive medium was developed to screen and isolate mutagenic Leuconostoc oenos strains defective in malolactic fermentation. The essential components of the medium included fructose (22 mM), l -malic acid (74.6 mM), bromocresol green (as pH indicator), and cellulose powder. The wild-type colonies turned blue, but defective malolactic colonies gave an acid reaction and remained yellow-green.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference22 articles.

1. Malolactic enzyme of Lactobacillus plantarum;Caspritz G.;J. Biol. Chem.,1983

2. Plasmid profiles in Leuconostoc species;Cavin J. F.;Microbiol. Alim. Nutr.,1988

3. An evaluation of two commercial Leuconostoc oenos strains for induction of malolactic fermentation under winery conditions;Costello P. J.;Food Technol. Aust.,1985

4. Numbers and species of lactic acid bacteria in wines during vinification;Costello P. J.;Food Technol. Aust.,1983

5. Mutation and selection of Lactobacillus plantarum strains that do not produce carbon dioxide from malate;Daeschel M. A.;Appl. Environ. Microbiol.,1984

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