Affiliation:
1. Department of Biology, University of California, San Diego, La Jolla, California 92037
Abstract
Two methods were devised to select a series of overlapping deletion mutations carried on episomal elements in
Escherichia coli
. The deletions were then used in an analysis of (i) the relative position on the genome of previously described mutant loci in the flagellar genes, (ii) the relative position of a newly defined cistron,
flaN
, and (iii) the orientation and direction of transcription of genes previously assigned to multicistronic transcriptional units. As a result of this analysis and previous work, we report the following arrangement of the flagellar genes in the
his-aroD
region of the
E. coli
genetic map:
his-flaR-flaQ-flaP-flaA-flaE-flaO-flaC-flaB-flaN-hag-flaD-uvrC-flaI-mot- flaG-flaH
. The genes
flaA, P, Q
, and
R, flaB, C, O
and
E
, and
flaG
and
H
are co-transcribed in that order.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
26 articles.
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