Affiliation:
1. Naval Biomedical Research Laboratory, School of Public Health, University of California, Berkeley, California 94625
Abstract
The indirect fluorescent-antibody test (IFAT), employing treated and standardized antiserum in a single pool, was earlier reported to have been used successfully for the preliminary identification of nine respiratory viruses in second to ninth passage multiplying in cells propagated on microscope slides. This report describes parameters affecting the isolation in first passage and the identification by IFAT of adenovirus types 4 and 7 and coxsackievirus type A21 present in stored clinical specimens inoculated into microcultures of WI-38 cells. Isolation frequency was comparable to that obtained in tube cultures, and identification by IFAT of viruses in microcultures could be accomplished in 3 to 4 hr after recognition of a cytopathogenic effect.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference8 articles.
1. Rapid detection and identification of respiratory viruses by direct immunofluorescence. Appi;D'Alessio E.;Microbiol.,1970
2. Acute respiratory disease associated with coxsackie A-21 virus infection;Johnson K. M.;J. Amer. Med. Ass.,1962
3. A transport medium for diagnostic virology;Leibovitz A.;Proc. Soc. Exp. Biol. Med.,1969
4. Multiplication and cytopathology of coxsackie A-21 virus in rotated and stationary tissue cultures;Mufson M. A.;Proc. Soc. Exp. Biol. Med.,1962
5. Immunologic identification of coxsackie A-21 virus with Coe virus;Schmidt N. J.;Proc. Soc. Exp. Biol. Med.,1961
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