Design and Construction of an Apparatus for the Growth of Micro Cell Cultures on Standard Glass Microscope Slides and Its Application for Screening Large Numbers of Sera by the Indirect Fluorescent Antibody Technique

Author:

Lawman Michael J. P.1,Caie Ian S.1

Affiliation:

1. Animal Virus Research Institute, Pirbright, Woking, Surrey, GU24 ONF England

Abstract

A simple stainless-steel apparatus was designed to contain standard microscope slides on which were grown micro cell cultures in the form of 16 individual monolayers per slide. The application of this apparatus for the screening of serum samples by fluorescent antibody techniques is described.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference10 articles.

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2. Miniature tissue culture technique with a modified plastic microplate;Hernandez-Baumgarten E. M.;Appl. Microbiol.,1972

3. Polyoma transformation of hamster cell clones-an investigation of genetic factors affecting cell competence;Macpherson I. A.;Virology,1962

4. Studies on the fluorescent and neutralizing antibodies to bluetongue virus in sheep;Pini A. H.;Arch. Gesamte Virusforsch.,1968

5. Use of vinyl plastic containers in tissue cultures for virus assays;Rightsel W. E.;J. Immunol.,1956

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