Microtissue Culture Test for the Titration of Low Concentrations of Diphtheria Antitoxin in Minimal Amounts of Human Sera
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Published:1973-01
Issue:1
Volume:25
Page:1-4
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ISSN:0003-6919
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Container-title:Applied Microbiology
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language:en
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Short-container-title:Appl Microbiol
Author:
Quevillon Marc1,
Chagnon André1
Affiliation:
1. Institut de Microbiologie et d'Hygiène de l'Université de Montréal, Ville de Laval, Québec, Canada
Abstract
A microtissue culture method for the assay of low concentrations of diphtheria antitoxin in human sera has been developed, using a monkey kidney cell (VERO) culture technique. Results obtained with sera from nonvaccinated children and with immune sera from children vaccinated with three and four injections of diphtheria pertussis tetanus vaccine were in agreement with antitoxin levels considered necessary to denote immunity to diphtheria. The use of microplates and organic buffer for culturing the animal cells improved the stability of the tissue culture system. The described method is sensitive, economical, and applicable for the titration of antitoxin in human sera particularly from infants and children from whom a minimum amount of serum is available.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference13 articles.
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