Evaluation of a Rapid and Accessible Reverse Transcription-Quantitative PCR Approach for SARS-CoV-2 Variant of Concern Identification-Reference-Cited by-同舟云学术

Evaluation of a Rapid and Accessible Reverse Transcription-Quantitative PCR Approach for SARS-CoV-2 Variant of Concern Identification

Published:2022-05-18 Issue:5 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Yeung Priscilla S.-W.¹,Wang Hannah¹^ORCID,Sibai Mamdouh¹,Solis Daniel¹,Yamamoto Fumiko¹,Iwai Naomi²,Jiang Becky²,Hammond Nathan³,Truong Bernadette²,Bihon Selamawit²,Santos Suzette²,Mar Marilyn²,Mai Claire³,Mfuh Kenji O.²,Miller Jacob A.⁴,Huang ChunHong¹,Sahoo Malaya K.¹,Zehnder James L.¹,Pinsky Benjamin A.¹²⁵^ORCID

Affiliation:

1. Department of Pathology, Stanford University School of Medicine, Stanford, California, USA

2. Clinical Virology Laboratory, Stanford Health Care, Stanford, California, USA

3. Clinical Genomics Laboratory, Stanford Health Care, Stanford, California, USA

4. Department of Radiation Oncology, Stanford University School of Medicine, Stanford, California, USA

5. Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA

Abstract

The ability to distinguish between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) is of ongoing interest due to differences in transmissibility, responses to vaccination, clinical prognosis, and therapy. Although detailed genetic characterization requires whole-genome sequencing (WGS), targeted nucleic acid amplification tests can serve a complementary role in clinical settings, as they are more rapid and accessible than sequencing in most laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.00178-22

Reference43 articles.

1. World Health Organization. 2022. Tracking SARS-CoV2 variants. https://www.who.int/en/activities/tracking-SARS-CoV-2-variants. Accessed 28 February 2022.

2. The biological and clinical significance of emerging SARS-CoV-2 variants

3. Nextstrain: real-time tracking of pathogen evolution

4. On the evolutionary epidemiology of SARS-CoV-2

5. Estimated transmissibility and impact of SARS-CoV-2 lineage B.1.1.7 in England

Cited by 14 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis;Scientific Reports;2023-12-07

2. Comparison of nucleocapsid antigen with strand-specific reverse-transcription PCR for monitoring SARS-CoV-2 infection;Journal of Clinical Virology;2023-07

3. Development and application of an RT‒PCR assay for the identification of the delta and omicron variants of SARS-COV-2;Heliyon;2023-06

4. Evaluation of a multiplexed oligonucleotide ligation assay for SARS-CoV-2 variant identification;Journal of Clinical Virology;2023-05

5. Multiplex Assays Enable Simultaneous Detection and Identification of SARS-CoV-2 Variants of Concern in Clinical and Wastewater Samples;ACS Measurement Science Au;2023-04-21