Rational Design of Stapled Antimicrobial Peptides to Enhance Stability and <i>In Vivo</i> Potency against Polymicrobial Sepsis-Reference-Cited by-同舟云学术

Rational Design of Stapled Antimicrobial Peptides to Enhance Stability and In Vivo Potency against Polymicrobial Sepsis

Published:2023-04-13 Issue:2 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Yeh Jih-Chao¹,Hazam Prakash Kishore¹,Hsieh Chu-Yi¹,Hsu Po-Hsien²,Lin Wen-Chun¹,Chen Yun-Ru³,Li Chao-Chin⁴,Chen Jyh-Yih¹⁵^ORCID

Affiliation:

1. Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Jiaushi, Ilan, Taiwan

2. Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan

3. Academia Sinica Protein Clinic, Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

4. Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan

5. The iEGG and Animal Biotechnology Center and the Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan

Abstract

In our in vitro studies, one dual stapled peptide out of the series of candidates (TP4-3: FIIXKKSXGLFKKKAGAXKKKXIKK ) showed significant activity, low toxicity, and high stability (in 50% human serum). When tested in cecal ligation and puncture (CLP) mouse models of polymicrobial sepsis, TP4-3 improved survival (87.5% on day 7).

Funder

Marine Research Station, Academia Sinica, Taiwan

Higher Education Sprout Project Ministry of Education

Ministry of Science and Technology

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03853-22

Reference59 articles.

1. A Brief History of the Antibiotic Era: Lessons Learned and Challenges for the Future