Culture-Independent Workflow for Nanopore MinION-Based Sequencing of Influenza A Virus-Reference-Cited by-同舟云学术

Culture-Independent Workflow for Nanopore MinION-Based Sequencing of Influenza A Virus

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Miah Mojnu¹,Hossain Mohammad Enayet¹^ORCID,Hasan Rashedul¹,Alam Md Shaheen¹,Puspo Joynob Akter¹,Hasan Md Mahmudul¹,Islam Ariful²³,Chowdhury Sukanta¹,Rahman Mohammed Ziaur¹

Affiliation:

1. Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh

2. EcoHealth Alliance, New York, New York, USA

3. Centre for Integrative Ecology, School of Life and Environmental Science, Deakin University, Burwood, Victoria, Australia

Abstract

The Nanopore MinION-based influenza sequencing approach we are proposing makes it possible to sequence the influenza A virus, irrespective of its diverse serotypes, directly from clinical and environmental swab samples, so that we are not limited to virus culture. This third-generation, portable, multiplexing, and real-time sequencing strategy is highly convenient for local sequencing, particularly in low- and middle-income countries like Bangladesh.

Funder

HHS | Centers for Disease Control and Prevention

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.04946-22

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