Affiliation:
1. Department of Microbiology, Western Reserve University, Cleveland, Ohio
2. Department of Pathology, Western Reserve University, Cleveland, Ohio
Abstract
Young
, F. E. (Western Reserve University Cleveland, Ohio)
and John Spizizen
. Incorporation of deoxyribonucleic acid in the
Bacillus subtilis
transformation system. J. Bacteriol.
86:
392–400. 1963.—The optimal conditions for the incorporation of deoxyribonucleic acid (DNA) were studied. In competent cells, the irreversible binding of DNA was influenced by temperature, hydrogen ion concentration, and aeration. Divalent cations, such as barium, strontium, calcium, or magnesium, were required. Under suboptimal environmental conditions and with metabolic inhibitors, the process of transformation was decreased to a greater extent than was incorporation of DNA. Under conditions of phosphate depletion, the incorporation of P
32
increased. However, the frequency of transformation decreased. This inducible process was not related to competence.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference25 articles.
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3. Cation effects on the denaturation of DNA;DOVE W. F.;J. Mol. Biol.,1962
4. Metal ions as stabilizers or destabilizers of the deoxyribonucleic acid structure;EICHHORN G. L.;Nature,1962
5. L'etat du DNA transformant au cours des premieres phases de la transformation bacterienne;EPHRUSSI-TAYLOR H.;Compt. Rend. Soc. Biol.,1960
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