Characterization of Mono- and Mixed-Culture Campylobacter jejuni Biofilms

Author:

Ica Tuba12,Caner Vildan3,Istanbullu Ozlem2,Nguyen Hung Duc2,Ahmed Bulbul2,Call Douglas R.4,Beyenal Haluk2

Affiliation:

1. Dumlupinar University, Faculty of Art and Science, Department of Biology, Kutahya, Turkey

2. The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA

3. Pamukkale University, Faculty of Medicine, Department of Medical Biology, Denizli, Turkey

4. Department of Veterinary Microbiology and Pathology and the Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA

Abstract

ABSTRACT Campylobacter jejuni , one of the most common causes of human gastroenteritis, is a thermophilic and microaerophilic bacterium. These characteristics make it a fastidious organism, which limits its ability to survive outside animal hosts. Nevertheless, C. jejuni can be transmitted to both humans and animals via environmental pathways, especially through contaminated water. Biofilms may play a crucial role in the survival of the bacterium under unfavorable environmental conditions. The goal of this study was to investigate survival strategies of C. jejuni in mono- and mixed-culture biofilms. We grew monoculture biofilms of C. jejuni and mixed-culture biofilms of C. jejuni with Pseudomonas aeruginosa . We found that mono- and mixed-culture biofilms had significantly different structures and activities. Monoculture C. jejuni biofilms did not consume a measurable quantity of oxygen. Using a confocal laser scanning microscope (CLSM), we found that cells from monoculture biofilms were alive according to live/dead staining but that these cells were not culturable. In contrast, in mixed-culture biofilms, C. jejuni remained in a culturable physiological state. Monoculture C. jejuni biofilms could persist under lower flow rates (0.75 ml/min) but were unable to persist at higher flow rates (1 to 2.5 ml/min). In sharp contrast, mixed-culture biofilms were more robust and were unaffected by higher flow rates (2.5 ml/min). Our results indicate that biofilms provide an environmental refuge that is conducive to the survival of C. jejuni .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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