Affiliation:
1. Department of Microbiology, University of Nigeria, Nsukka, Nigeria
Abstract
Anaerobic and facultatively anaerobic bacteria associated with soft rot of yam (
Diascorea rotundata
) were isolated by the looping-out method and found to consist of
Clostridium
(three isolates),
Corynebacterium
(three isolates),
Vibrio
(one isolate), and
Bacillus lentus
(one isolate). Enzyme assay for hydrolase, lyase, and pectinesterase activities by the cup-plate method showed that except for
Vibrio
sp.,
B. lentus
, and two isolates of
Corynebacterium
no pectinase activity could be detected for organisms cultured on pectin medium. Most of the cultures on yam tissue, however, showed activities for the three enzymes. The viscometric assay for hydrolase and lyase enzymes indicated a significant level of hydrolase activity (a 40.90% decrease in viscosity for
Vibrio
sp. and
Corynebacterium
spp.), but no lyase activity for most of the isolates. Two isolates of
Corynebacterium
and
B. lentus
caused changes in fresh yams suggestive of soft rot.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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