Affiliation:
1. Université des Sciences et Techniques de Lille, Villeneuve d'Ascq, France.
Abstract
We investigated the structures of the exopolysaccharides (EPSs) produced by Streptococcus thermophilus SFi39 and SFi12. Both polymers were found to have molecular masses of greater than 2 x 10(6) Da. The SFi39 EPS consisted of D-glucose and D-galactose in a molar ratio of 1:1, whereas the SFi12 EPS was composed of D-galactose, L-rhamnose, and D-glucose in a molar ratio of 3:2:1. Methylation analysis of and nuclear magnetic resonance spectra recorded from the native polysaccharide, as well as oligosaccharides released by partial acid hydrolysis, allowed the complete structural determination of the SFi39 EPS, which consists of the following tetrasaccharide repeating unit: [formula: see text] Similar spectra recorded only from the native polysaccharide were sufficient to allow the structural determination of the SFi12 EPS, which consists of the following hexasaccharide repeating unit: [formula: see text] This study shows that the texturizing properties of different S. thermophilus ropy strains are based on the production of EPSs exhibiting chemical similarities but structural differences.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference30 articles.
1. Exocellular polysaccharides produced by lactic acid bacteria;Cerning J.;FEMS Microbiol. Rev.,1990
2. A rapid and simple method for the permethylation of carbohydrates;Ciucanu I.;Carbohydr. Res.,1984
3. Doco T. B. Fournet D. Carcano P. Ramos A. Loones J. M. Piot and D. Guillochon. September 1989. Polysaccharide application comme agent épaississant et comme agent antitumoral. European patent 331 564.
4. Structure of an exocellular polysaccharide produced by Streptococcus thermophilus;Doco T.;Carbohydr. Res.,1990
5. Colorimetric method for determination of sugars and related substances;Dubois M. A.;Anal. Chem.,1956
Cited by
96 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献