Affiliation:
1. Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
Abstract
We have explored the potential of using the apramycin resistance gene as a marker in mycobacterial gene transfer studies. Shuttle plasmids available for both electroporation and conjugation studies have been constructed, and we have successfully validated the use of the apramycin resistance gene as a component of cloning vectors for Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
32 articles.
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