Next Generation of Tn 7 -Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii-Reference-Cited by-同舟云学术

Next Generation of Tn 7 -Based Single-Copy Insertion Elements for Use in Multi- and Pan-Drug-Resistant Strains of Acinetobacter baumannii

Published:2019-06 Issue:11 Volume:85 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ducas-Mowchun Kaleigh¹,De Silva P. Malaka¹,Crisostomo Leandro¹,Fernando Dinesh M.¹,Chao Tzu-Chiao²,Pelka Peter¹,Schweizer Herbert P.³,Kumar Ayush¹⁴^ORCID

Affiliation:

1. Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

2. Department of Biology, University of Regina, Regina, Saskatchewan, Canada

3. University of Florida, College of Medicine, Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, Gainesville, Florida, USA

4. Manitoba Chemosensory Biology Group, University of Manitoba, Winnipeg, Manitoba, Canada

Abstract

Acinetobacter baumannii is a high-priority pathogen for which research on mechanisms of resistance and virulence is a critical need. Commonly used antibiotic selection markers are not suitable for use in MDR and XDR isolates of A. baumannii due to the high antibiotic resistance of these isolates, which poses a barrier to the study of this pathogen. This study demonstrates the practical potential of using apramycin and zeocin mini-Tn 7 - and Flp recombinase-encoded constructs to carry out genomic manipulations in clinical isolates of A. baumannii displaying MDR and XDR phenotypes.

Funder

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00066-19

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