Development and Clinical Evaluation of an Internally Controlled, Single-Tube Multiplex Real-Time PCR Assay for Detection of Legionella pneumophila and Other Legionella Species-Reference-Cited by-同舟云学术

Development and Clinical Evaluation of an Internally Controlled, Single-Tube Multiplex Real-Time PCR Assay for Detection of Legionella pneumophila and Other Legionella Species

Published:2003-09 Issue:9 Volume:41 Page:4016-4021
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Templeton Kate E.¹,Scheltinga Sitha A.¹,Sillekens Peter²,Crielaard Jantine W.¹,van Dam Alje P.¹,Goossens Herman¹³,Claas Eric C. J.¹

Affiliation:

1. Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden

2. BioMerieux, Boxtel, The Netherlands

3. Department of Microbiology, University of Antwerp, Antwerp, Belgium

Abstract

ABSTRACT A multiplex real-time PCR assay for detection of Legionella pneumophila and Legionella spp. and including an internal control was designed. Legionella species, L. pneumophila , and the internal control were detected simultaneously by probes labeled with 6-carboxy-fluorescein, hexachlorofluorescein, and indodicarbocyanine, respectively. Therefore, no postamplification analysis was required in order to distinguish the targets. The sensitivity of both assays was 2.5 CFU/ml, and from analysis of 10 culture-positive and 74 culture-negative samples from patients investigated for legionellosis, 100% agreement was observed by both assays in comparison to culture. Four additional positives were found by the multiplex real-time PCR assay in the Legionella culture-negative samples.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.9.4016-4021.2003

Reference28 articles.

1. Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay

2. Breiman, R. F., and J. C. Butler. 1998. Legionnaires' disease: clinical, epidemiological, and public health perspectives. Semin. Respir. Infect.13:84-89.

3. Detection of Legionella Species in Respiratory Specimens Using PCR with Sequencing Confirmation

4. Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR

5. Comparison of the Binax Legionella Urinary Antigen Enzyme Immunoassay (EIA) with the Biotest Legionella Urin Antigen EIA for Detection of Legionella Antigen in both Concentrated and Nonconcentrated Urine Samples

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