Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay-Reference-Cited by-同舟云学术

Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay

Published:2000-11 Issue:11 Volume:38 Page:4215-4218
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ballard A. L.¹,Fry N. K.²,Chan L.³,Surman S. B.³,Lee J. V.³,Harrison T. G.²,Towner K. J.¹

Affiliation:

1. Department of Microbiology and PHLS Laboratory, University Hospital,1 and

2. Respiratory and Systemic Infection Laboratory, PHLS Central Public Health Laboratory, London NW9 5HT,2 United Kingdom

3. PHLS Water and Environmental Microbiology Research Unit,3 Queen's Medical Centre, Nottingham NG7 2UH, and

Abstract

ABSTRACT A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.11.4215-4218.2000

Reference17 articles.

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