Sec-Dependent Secretion of Subtilase SptE in <i>Haloarchaea</i> Facilitates Its Proper Folding and Heterocatalytic Processing by Halolysin SptA Extracellularly-Reference-Cited by-同舟云学术

Sec-Dependent Secretion of Subtilase SptE in Haloarchaea Facilitates Its Proper Folding and Heterocatalytic Processing by Halolysin SptA Extracellularly

Published:2022-04-26 Issue:8 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Mei Sha¹,Li Moran¹,Sun Yiqi¹,Deng Xi¹,Chen Nifan¹,Liu Yang¹,Yin Jing¹,Luo Hongyi¹,Wu Yi²,He Dan²,Gan Fei¹,Tang Bing²³,Tang Xiao-Feng¹³^ORCID

Affiliation:

1. Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China

2. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China

3. Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan, China

Abstract

While Tat-dependent haloarchaeal subtilases (halolysins) have been extensively studied, the information about Sec-dependent subtilases of haloarchaea is limited. Our results demonstrate that proper maturation of Sec-dependent subtilase SptE of Natrinema sp. strain J7-2 depends on the action of halolysin SptA from the same strain, yielding multiple hetero- and autocatalytic mature forms.

Funder

National Natural Science Foundation of China

National Infrastructure of Natural Resources for Science and Technology Program of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.00246-22

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