Dissecting the Arginine and Lysine Biosynthetic Pathways and Their Relationship in Haloarchaeon Natrinema gari J7-2 via Endogenous CRISPR-Cas System-Based Genome Editing

Author:

Wu Yi1,Zhang Jia2,Wang Bingxue1,Zhang Yanyan2,Li Huai2,Liu Yang1,Yin Jing1,He Dan2,Luo Hongyi2,Gan Fei13,Tang Bing23ORCID,Tang Xiao-Feng13ORCID

Affiliation:

1. Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China

2. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China

3. Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Wuhan, China

Abstract

Due to their roles in amino acid metabolism and close evolutionary relationship, arginine and lysine biosynthetic pathways represent interesting models for probing functional specialization of metabolic routes. The current knowledge with respect to arginine and lysine biosynthesis is limited for haloarchaea compared to that for bacteria and hyperthermophilic archaea.

Funder

MOST | National Natural Science Foundation of China

National Infrastructure of Natural Resources for Science and Technology Program of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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