Azole and Amphotericin B MIC Values against Aspergillus fumigatus : High Agreement between Spectrophotometric and Visual Readings Using the EUCAST EDef 9.3.2 Procedure-Reference-Cited by-同舟云学术

Azole and Amphotericin B MIC Values against Aspergillus fumigatus : High Agreement between Spectrophotometric and Visual Readings Using the EUCAST EDef 9.3.2 Procedure

Published:2020-12-16 Issue:1 Volume:65 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Serrano-Lobo Julia¹²,Gómez Ana¹²,Sánchez-Yebra Waldo³,Fajardo Miguel⁴,Lorenzo Belén⁵,Sánchez-Reus Ferrán⁶^ORCID,Vidal Inmaculada⁷,Fernández-Torres Marina⁸,Sánchez-Romero Isabel⁹,Ruiz de Alegría-Puig Carlos¹⁰,del Pozo José Luis¹¹,Muñoz Patricia¹²¹²¹³^ORCID,Escribano Pilar¹²^ORCID,Guinea Jesús¹²¹²^ORCID

Affiliation:

1. Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain

2. Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

3. Unidad de Microbiología, UGC Biotecnología, Complejo Hospitalario Torrecárdenas, Almería, Spain

4. Clinical Microbiology Department, Hospital Universitario de Badajoz, Badajoz, Spain

5. Clinical Microbiology Department, Hospital Río Hortega, Valladolid, Spain

6. Clinical Microbiology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain

7. Clinical Microbiology Department, Hospital General de Alicante, Alicante, Spain

8. Clinical Microbiology Department, Hospital Universitario de Álava-BIOARABA, Vitoria, Spain

9. Clinical Microbiology Department, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain

10. Clinical Microbiology Department, Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain

11. Clinical Microbiology and Infectious Diseases Department, Clínica Universidad de Navarra, Pamplona, Spain

12. CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain

13. Medicine Department, Faculty of Medicine, Universidad Complutense de Madrid, Madrid, Spain

Abstract

The EUCAST EDef 9.3.2 procedure recommends visual readings of azole and amphotericin B MICs against Aspergillus spp. Visual determination of MICs may be challenging. In this work, we aim to obtain and compare visual and spectrophotometric MIC readings of azoles and amphotericin B against Aspergillus fumigatus sensu lato isolates. A total of 847 A. fumigatus sensu lato isolates ( A. fumigatus sensu stricto [ n = 828] and cryptic species [ n = 19]) were tested against amphotericin B, itraconazole, voriconazole, posaconazole, and isavuconazole using the EUCAST EDef 9.

Funder

Fondo de Investigacion Sanitaria

Basilea Pharmaceutica

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01693-20

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