Pentatricopeptide Repeat Proteins in
Trypanosoma brucei
Function in Mitochondrial Ribosomes
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Published:2007-10
Issue:19
Volume:27
Page:6876-6888
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ISSN:0270-7306
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Container-title:Molecular and Cellular Biology
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language:en
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Short-container-title:Mol Cell Biol
Author:
Pusnik Mascha1, Small Ian2, Read Laurie K.3, Fabbro Thomas1, Schneider André1
Affiliation:
1. Department of Biology/Cell and Developmental Biology, University of Fribourg, CH-1700 Fribourg, Switzerland 2. ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Perth 6009, WA, Australia 3. Department Microbiology and Immunology, SUNY Buffalo School of Medicine, Buffalo, New York 14214
Abstract
ABSTRACT
The pentatricopeptide repeat (PPR), a degenerate 35-amino-acid motif, defines a novel eukaryotic protein family. Plants have 400 to 500 distinct PPR proteins, whereas other eukaryotes generally have fewer than 5. The few PPR proteins that have been studied have roles in organellar gene expression, probably via direct interaction with RNA. Here we show that the parasitic protozoan
Trypanosoma brucei
encodes 28 distinct PPR proteins, an extraordinarily high number for a nonplant organism. A comparative analysis shows that seven out of eight selected PPR proteins are mitochondrially localized and essential for oxidative phosphorylation. Six of these are required for the stabilization of mitochondrial rRNAs and, like ribosomes, are associated with the mitochondrial membranes. Furthermore, one of the PPR proteins copurifies with the large subunit rRNA. Finally, ablation of all of the PPR proteins that were tested induces degradation of the other PPR proteins, indicating that they function in concert. Our results show that a significant number of trypanosomal PPR proteins are individually essential for the maintenance and/or biogenesis of mitochondrial rRNAs.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
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