Rapid Detection of the Top Six Non-O157 Shiga Toxin-Producing Escherichia coli O Groups in Ground Beef by Flow Cytometry-Reference-Cited by-同舟云学术

Rapid Detection of the Top Six Non-O157 Shiga Toxin-Producing Escherichia coli O Groups in Ground Beef by Flow Cytometry

Published:2012-06 Issue:6 Volume:50 Page:2137-2139
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hegde Narasimha V.¹,Jayarao Bhushan M.¹,DebRoy Chitrita¹

Affiliation:

1. Escherichia coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA

Abstract

ABSTRACT Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10 3 target cells in a bacterial mixture of 10 5 CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00137-12

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