Affiliation:
1. Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan
2. Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan
Abstract
ABSTRACT
We investigated the efficacy of a loop-mediated isothermal amplification (LAMP) assay for detection of chicken meat samples naturally contaminated with
Campylobacter jejuni
and
Campylobacter coli
. A total of 144 Preston enrichment broth cultures from chicken meat samples were assessed by using the LAMP assay and conventional culture methods, which consist of a combination of Preston enrichment culturing and plating onto Butzler and modified charcoal cefoperazone deoxycholate agars. Compared with
C. jejuni
-
C. coli
isolation using the conventional culture test, the LAMP results showed 98.5% (67/68) and 97.4% (74/76) sensitivity and specificity, respectively, and the positive and negative predictive values were 97.1% (67/69) and 98.7% (74/75), respectively. The conventional culture test required more than 3 to 4 days to isolate and identify
C. jejuni
and
C. coli
in the Preston enrichment cultures. In contrast, the LAMP assay was markedly faster, requiring less than 90 min from the beginning of DNA extraction to final detection and differentiation of
C. jejuni
and
C. coli
. In total, the LAMP assay required 23.5 to 25.5 h from the beginning of the enrichment culture to final determination. These results suggest that our LAMP assay is a powerful tool for rapid, sensitive, and practical detection of
C. jejuni
and
C. coli
which may facilitate surveillance and control of
C. jejuni-C. coli
contamination in chicken, as well as investigations of food poisoning incidents caused by these organisms. This is the first report of a highly sensitive and specific LAMP assay to detect and differentiate
C. jejuni
and
C. coli
in chicken meat samples.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology