Author:
Caro-Castro Junior,Orellana-Peralta Fiorella,Flores-Leon Diana,Cuch-Meza Evans,Gavilán Ronnie G.,Quino Willi
Abstract
AbstractIn recent years, the worldwide incidence of diarrheal diseases caused byCampylobacter jejunihas been increasing, causing large-scale outbreaks in developing countries, which added to the capacity of the microorganism to cause Guillain-Barré syndrome (GBS), increasing the need for rapid and timely diagnosis to stop and prevent outbreaks. In this study, a loop-mediated isothermal amplification (LAMP) assay based on thecdtCgene was developed and evaluated for the rapid detection ofC. jejuni, using 91 strains for the standardization and validation. The LAMP assay was compared to whole-genome sequencing as the gold standard. The LAMP assay forC. jejunishowed a sensitivity of 100% (CI: 90.94 - 100%), a specificity of 100% (CI: 89.56 - 100%), a positive predictive value of 100% (CI: 90.94 - 100%) and a negative predictive value of 100% (CI: 89.56 - 100%). The assay demonstrated strong agreement between the LAMP assay and genomic sequencing (kappa value = 1). The LAMP assay based on thecdtCgene is a method that provides reliable and rapid results, with high sensitivity and specificity for the identification ofC. jejuni, and is considered a suitable alternative for the diagnosis of diarrheal infections by this pathogen in low-income countries.
Publisher
Cold Spring Harbor Laboratory
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