Affiliation:
1. Department of Biochemistry and the Molecular and Cellular Biology Program, Division of Biological Sciences, State University of New York, Stony Brook, New York 11790
Abstract
The characteristics of the inducible galactose system in
Saccharomyces cerevisiae
were studied by using the nonmetabolized galactose analogues,
l
-arabinose and
d
-fucose, and galactokinaseless and transportless mutants. Induced wild-type cells transport
l
-arabinose by facilitated diffusion. Transportless cells transport neither galactose nor
l
-arabinose above the noninduced rate, whereas galactokinaseless cells transport galactose
l
-arabinose and
d
-fucose by facilitated diffusion. Determination of unidirectional rate of
14
C-labeled galactose uptake by preloaded galactokinaseless cells, containing a large unlabeled free-galactose pool, showed that the rate of galactose uptake by facilitated diffusion is greater than the rate of galactose metabolism at similar external galactose concentrations.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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