Affiliation:
1. Microbiology, School of Life and Health Sciences, Aston University, Aston Triangle. Birmingham, United Kingdom
Abstract
ABSTRACT
The mechanisms by which bacteria resist killing by antibiotics and biocides are still poorly defined, although repeated exposure to sublethal concentrations of antibacterial agents undoubtedly contributes to their development. This study aimed both to investigate the potential of
Salmonella enterica
and
Escherichia coli
O157 for adaptive resistance to commonly used biocides and to determine any cross-resistance to antibiotics. Strains were repeatedly passaged in media containing increasing concentrations of a biocide or antibiotic until adaptive resistance was obtained. A wide panel of antimicrobial agents was then screened by using the adapted strain to determine cross-resistance, if any. Adaptive resistance was readily achieved for both
S. enterica
and
E. coli
O157. Cross-resistance in adaptively resistant
S. enterica
varied with the serotype;
Salmonella enterica
serovar Enteritidis expressed cross-resistance to chloramphenicol, whereas
Salmonella enterica
serovar Typhimurium expressed cross-resistance to chlorhexidine. Benzalkonium chloride-resistant
Salmonella enterica
serovar Virchow showed elevated resistance to chlorhexidine; however, chlorhexidine-resistant
Salmonella
serovar Virchow did not demonstrate reciprocal cross-resistance to benzalkonium chloride, suggesting specific rather than generic resistance mechanisms.
E. coli
O157 strains acquired high levels of resistance to triclosan after only two sublethal exposures and, when adapted, repeatedly demonstrated decreased susceptibilities to various antimicrobial agents, including chloramphenicol, erythromycin, imipenem, tetracycline, and trimethoprim, as well as to a number of biocides. These observations raise concern over the indiscriminate and often inappropriate use of biocides, especially triclosan, in situations where they are unnecessary, whereby they may contribute to the development of microbial resistance mechanisms.
Publisher
American Society for Microbiology
Reference29 articles.
1. Adrian, P. V., M. Plessis, K. P. Klugman, and S. G. B. Amyes. 1998. New trimethoprim-resistant dihydrofolate reductase cassette dfrXV, inserted in a class 1 integron. J. Antimicrob. Agents42:2221-2224.
2. Beumer R. S. F. Bloomfield M. Exner G. M. Fara K. J. Nath and E. Scott. September 2000. Microbial resistance and biocides. IFH review. [Online.] International Scientific Forum on Home Hygiene Geneva Switzerland. http://www.ifh-homehygiene.org/forum/antresFINAL.pdf
.
3. Braid, J. J., and M. C. J. Wale. 2002. The antibacterial activity of triclosan-impregnated storage boxes against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus and Shewanella putrefaciens in conditions simulating domestic use. J. Antimicrob. Chemother.49:87-94.
4. British Society for Antimicrobial Chemotherapy. 1991. Journal of Antimicrobial Chemotherapy vol. 27 suppl. D. Guide to sensitivity testing. Academic Press London United Kingdom.
5. Carsenti-Etesse, H., P.-M. Roger, B. Dunais, S. Durgeat, G. Mancini, M. Bensoussan, and P. Dellamonica. 1999. Gradient plate method to induce Streptococcus pyogenes resistance. J. Antimicrob. Chemother.44:439-443.
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