New Trimethoprim-Resistant Dihydrofolate Reductase Cassette, dfrXV , Inserted in a Class 1 Integron-Reference-Cited by-同舟云学术

New Trimethoprim-Resistant Dihydrofolate Reductase Cassette, dfrXV , Inserted in a Class 1 Integron

Published:1998-09 Issue:9 Volume:42 Page:2221-2224
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Adrian Peter V.¹,du Plessis Mignon¹,Klugman Keith P.¹,Amyes Sebastian G. B.²

Affiliation:

1. Department of Medical Microbiology, University of the Witwatersrand, and the South African Institute for Medical Research, Johannesburg, South Africa,1 and

2. Molecular Chemotherapy, Department of Medical Microbiology, University of Edinburgh, Edinburgh, United Kingdom2

Abstract

ABSTRACT The nucleotide sequence of a plasmid-borne trimethoprim resistance gene from a commensal fecal Escherichia coli isolate revealed a new dihydrofolate reductase gene, dfrXV , which occurred as a gene cassette integrated in a site-specific manner in a class 1 integron. The new gene shows 84% nucleotide identity and the predicted protein shows 90% amino acid identity with dfrI and DHFR type I, respectively. Genes for spectinomycin resistance, aadA1 [ ant (3′′)-Ia ], and sulfonamide resistance, sulI , were located downstream of dfrXV in a manner identical to that in pLMO229.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.42.9.2221

Reference32 articles.

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