Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Pseudomonas fluorescens Proteases in Ultrahigh-Temperature-Treated Milk-Reference-Cited by-同舟云学术

Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Pseudomonas fluorescens Proteases in Ultrahigh-Temperature-Treated Milk

Published:1990-04 Issue:4 Volume:56 Page:1188-1190
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Clements Richard S.¹,Wyatt David M.¹,Symons Michael H.¹,Ewings Kenneth N.¹

Affiliation:

1. Immunology Department, Queensland University of Technology, Brisbane, 4000, and Department of Primary Industries, Queensland Food Research Laboratories, Hamilton, 4007,2 Queensland, Australia

Abstract

An inhibition enzyme-linked immunosorbent assay was developed to detect low levels of the proteases extracted from four strains of Pseudomonas fluorescens. The assay detected between 0.24 and 7.8 ng of protease per ml of ultrahigh-temperature-treated milk and could be completed within 6 h. It could be used as a framework for a test system for quantifying spoilage proteases in dairy products.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.56.4.1188-1190.1990

Reference15 articles.

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