SOSIP Changes Affect Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Conformation and CD4 Engagement-Reference-Cited by-同舟云学术

SOSIP Changes Affect Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Conformation and CD4 Engagement

Published:2018-10 Issue:19 Volume:92 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Alsahafi Nirmin¹²,Anand Sai Priya¹²,Castillo-Menendez Luis³⁴,Verly Myriam Maude¹,Medjahed Halima¹,Prévost Jérémie¹⁵,Herschhorn Alon⁶,Richard Jonathan¹⁵,Schön Arne⁷,Melillo Bruno⁸,Freire Ernesto⁷,Smith Amos B.⁸,Sodroski Joseph³⁴,Finzi Andrés¹²⁵

Affiliation:

1. Centre de Recherche du CHUM, Montreal, Quebec, Canada

2. Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada

3. Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA

4. Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA

5. Department of Microbiology, Infectious Diseases and Immunology, Université de Montréal, Montreal, Quebec, Canada

6. Division of Infectious Diseases and International Medicine, Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA

7. Department of Biology, The Johns Hopkins University, Baltimore, Maryland, USA

8. Department of Chemistry, School of Arts and Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA

Abstract

The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) trimer is triggered by receptor binding to mediate the entry of the virus into cells. Most structural studies of Env trimers have utilized truncated soluble gp140 Envs stabilized with the I559P and SOS changes. Here we present evidence indicating that these stabilizing changes have a profound impact on the conformation of Env, moving Env away from the native pretriggered Env conformation. Our studies underscore the need to acquire structural information on the pretriggered Env conformation, which is recognized by most broadly reactive neutralizing antibodies.

Funder

HHS | National Institutes of Health

Canada Research Chairs

Gouvernement du Canada | Canadian Institutes of Health Research

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01080-18

Reference53 articles.

1. The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and Immunogens