Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions

Author:

Munro James B.1,Gorman Jason2,Ma Xiaochu1,Zhou Zhou3,Arthos James4,Burton Dennis R.56,Koff Wayne C.7,Courter Joel R.8,Smith Amos B.8,Kwong Peter D.2,Blanchard Scott C.3,Mothes Walther1

Affiliation:

1. Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA.

2. Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

3. Department of Physiology and Biophysics, Weill Cornell Medical College of Cornell University, New York, NY 10021, USA.

4. Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

5. Department of Immunology and Microbial Science, and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.

6. Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02129, USA.

7. International AIDS Vaccine Initiative (IAVI), New York, NY 10004, USA.

8. Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.

Abstract

HIV's shape-shifting envelope protein HIV's envelope protein (Env) coats virus particles and allows HIV to enter host cells. HIV entry is highly dynamic. Env proteins work in groups of three (called trimers), which bind to the viral receptor and co-receptor, both expressed by host cells. Viral receptor binding causes a structural rearrangement in the trimer that allows for co-receptor binding and finally, viral entry. To visualize dynamic changes in Env conformation during viral entry, Munro et al. added differently colored fluorescent tags to two different regions of individual HIV trimers. Single-molecule fluorescence resonance entry transfer revealed three distinct Env conformations before cell entry. Occupation of particular conformations depended on host receptor binding. Science , this issue p. 759

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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