Digalactoside Expression in the Lipopolysaccharide of Haemophilus influenzae and Its Role in Intravascular Survival-Reference-Cited by-同舟云学术

Digalactoside Expression in the Lipopolysaccharide of Haemophilus influenzae and Its Role in Intravascular Survival

Published:2005-10 Issue:10 Volume:73 Page:7022-7026
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Griffin Ruth¹,Bayliss Chris D.¹,Herbert Mark A.¹,Cox Andrew D.²,Makepeace Katherine¹,Richards James C.²,Hood Derek W.¹,Moxon E. Richard¹

Affiliation:

1. Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom

2. Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, KIA 0R6Canada

Abstract

ABSTRACT Digalactoside (galα-1-4 galβ) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A , lgtC , and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.73.10.7022-7026.2005

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