Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes

Author:

Metier Camille1234,Dow Jennifer5634,Wootton Hayley1234,Lynham Steven78193,Wren Brendan5634,Wagner Gerd K.101112134ORCID

Affiliation:

1. King's College London

2. Department of Chemistry

3. London

4. UK

5. London School of Hygiene and Tropical Medicine

6. Department of Infection Biology

7. Proteomics Facility

8. Centre of Excellence for Mass Spectrometry

9. The James Black Centre

10. Queen's University Belfast

11. School of Pharmacy

12. Medical Biology Centre

13. Belfast

Abstract

Labelling of proteins in Haemophilus influenzae with covalent, carbohydrate-based probes produced distinctive target profiles in lysates vs. intact cells.

Funder

King’s College London

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Reference39 articles.

1. J. Krysiak and S. A.Sieber , in Methods in Molecular Biology , ed. H. Overkleeft and B. Florea , Humana Press , New York , 2017 , vol. 1491 , pp. 57–74

2. S. Sharifzadeh , J. D.Shirley and E. E.Carlson , in Current Topics in Microbiology and Immunology , ed. B. F. Cravatt , K.-L. Hsu and E. Weerapana , Springer Nature , Switzerland , 2019 , vol. 420 , pp. 23–48

3. Activity-based protein profiling of microbes

4. New Electrophiles and Strategies for Mechanism-Based and Targeted Covalent Inhibitor Design

5. NHS-Esters As Versatile Reactivity-Based Probes for Mapping Proteome-Wide Ligandable Hotspots

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