Passive Protection against Anthrax by Using a High-Affinity Antitoxin Antibody Fragment Lacking an Fc Region-Reference-Cited by-同舟云学术

Passive Protection against Anthrax by Using a High-Affinity Antitoxin Antibody Fragment Lacking an Fc Region

Published:2005-12 Issue:12 Volume:73 Page:8362-8368
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Mabry Robert¹²,Rani Mridula²,Geiger Robert³,Hubbard Gene B.³,Carrion Ricardo⁴,Brasky Kathleen³,Patterson Jean L.⁴,Georgiou George²⁵,Iverson B. L.¹²

Affiliation:

1. Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas

2. Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas

3. Department of Comparative Medicine, Southwest Foundation for Biomedical Research, San Antonio, Texas

4. Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas

5. Department of Chemical Engineering and Biomedical Engineering, University of Texas at Austin, Austin, Texas

Abstract

ABSTRACT Passive immunization has been successfully employed for protection against bacterial and viral infections for over 100 years. Immunoglobulin Fc regions play a critical role in the clearance of bacterial pathogens by mediating antibody-dependent and complement-dependent cytotoxicity. Here we show that antibody fragments engineered to recognize the protective antigen component of the B. anthracis exotoxin with high affinity and conjugated to polyethylene glycol (PEG) for prolonged circulation half-life confer significant protection against inhalation anthrax despite their lack of Fc regions. The speed and lower manufacturing cost of bacterially expressed PEGylated antibody fragments could provide decisive advantages for anthrax prophylaxis. Importantly, our results suggest that PEGylated antibody fragments may represent a unique approach for mounting a rapid therapeutic response to emerging pathogen infections.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.73.12.8362-8368.2005

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