Affiliation:
1. U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701
Abstract
Improved culture conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of
Bacillus anthracis
holotoxin antigens. Levels of these antigens up to fivefold greater than the highest previously reported values were recovered with the described system. Cultures of Sterne, V770-NP1-R, and Vollum 1B strains of
B. anthracis
were monitored for growth, pH change, glucose utilization, supernatant protein concentration, lethal toxin activity, and protease activity.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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