Affiliation:
1. Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., 1-13-5, Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan
Abstract
ABSTRACT
The food-grade yeast
Candida utilis
has been engineered to confer a novel biosynthetic pathway for the production of carotenoids such as lycopene, β-carotene, and astaxanthin. The exogenous carotenoid biosynthesis genes were derived from the epiphytic bacterium
Erwinia uredovora
and the marine bacterium
Agrobacterium aurantiacum
. The carotenoid biosynthesis genes were individually modified based on the codon usage of the
C. utilis
glyceraldehyde 3-phosphate dehydrogenase gene and expressed in
C. utilis
under the control of the constitutive promoters and terminators derived from
C. utilis
. The resultant yeast strains accumulated lycopene, β-carotene, and astaxanthin in the cells at 1.1, 0.4, and 0.4 mg per g (dry weight) of cells, respectively. This was considered to be a result of the carbon flow into ergosterol biosynthesis being partially redirected to the nonendogenous pathway for carotenoid production.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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