METABOLIC FUNCTION OF BRANCHED-CHAIN VOLATILE FATTY ACIDS, GROWTH FACTORS FOR RUMINOCOCCI II

Abstract

Allison , M. J. (Dairy Cattle Research Branch, USDA, Beltsville, Md.), M. P. Bryant, I. Katz, and M. Keeney . Metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. II. Biosynthesis of higher branched-chain fatty acids and aldehydes. J. Bacteriol. 83: 1084–1093. 1962.—A number of strains of rumen bacteria require branched-chain volatile fatty acids for growth. A strain of Ruminococcus flavefaciens that requires either isovalerate or isobutyrate incorporates radioactive carbon from isovalerate-1-C 14 and isovalerate-3-C 14 into leucine and into the lipid fraction of the cells. Evidence obtained by both paper and gas chromatography indicated that most of the label in the lipid of cells grown in isovalerate-1-C 14 was in a branched-chain 15-carbon fatty acid, with some in a 17-carbon acid; about 7.5% of the C 14 was recovered in a branched-chain 15-carbon aldehyde. The aldehydes were in the phospholipid fraction and were presumably present as plasmalogen. A strain of R. albus was shown to require isobutyrate, 2-methyl- n -butyrate, or 2-ketoisovalerate for growth. This strain did not incorporate appreciable C 14 from isovalerate-1-C 14 or isovalerate-3-C 14 . When grown in a medium containing isobutyrate-1-C 14 , most of the cellular C 14 was found in the lipid fraction. Analysis of the lipid demonstrated that the label was present mainly as branched-chain 14-carbon and 16-carbon fatty acids, with 11% of the C 14 present in 14- and 16-carbon carbonyl compounds, presumably branched-chain aldehydes. Branched-chain 14-, 15-, and 16-carbon fatty acids are major components of the lipids of these rumen bacteria. The possibility that these acids and aldehydes, which are found in ruminant body and milk lipids, may be of microbial origin is discussed.