UV Light Inactivation of Mycobacterium avium subsp. paratuberculosis in Milk as Assessed by FASTPlaque TB Phage Assay and Culture-Reference-Cited by-同舟云学术

UV Light Inactivation of Mycobacterium avium subsp. paratuberculosis in Milk as Assessed by FASTPlaque TB Phage Assay and Culture

Published:2007-06 Issue:11 Volume:73 Page:3728-3733
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Altic Leslie C.¹,Rowe Michael T.²,Grant Irene R.¹

Affiliation:

1. Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast

2. Agri-Food and Biosciences Institute for Northern Ireland, Belfast, Northern Ireland, United Kingdom

Abstract

ABSTRACT UV light inactivation of Mycobacterium avium subsp. paratuberculosis in Middlebrook 7H9 broth and whole and semiskim milk was investigated using a laboratory-scale UV machine that incorporated static mixers within UV-penetrable pipes. UV treatment proved to be less effective in killing M. avium subsp. paratuberculosis suspended in milk (0.5- to 1.0-log 10 reduction per 1,000 mJ/ml) than that suspended in Middlebrook 7H9 broth (2.5- to 3.3-log 10 reduction per 1,000 mJ/ml). The FASTPlaque TB phage assay provided more rapid enumeration of surviving M. avium subsp. paratuberculosis (within 24 h) than culture on Herrold's egg yolk medium (6 to 8 weeks). Despite the fact that plaque counts were consistently 1 to 2 log 10 lower than colony counts throughout the study, UV inactivation rates for M. avium subsp. paratuberculosis derived using the phage assay and culture results were not significantly different ( P = 0.077).

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00057-07

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