Affiliation:
1. World Health Organization Collaborating Center for Reference and Research in Treponematoses, Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Abstract
Treponema pallidum
were extracted from testicular syphilomas of corticosteroid-treated rabbits and purified by differential centrifugation. The steroid therapy allowed a longer holding time for infected rabbits, which produced greater treponeme yields, averaging 1.58 × 10
10
treponemes per rabbit. The treatment, which also diminished cellular infiltration and increased the extracellular mucoid material in lesions, produced much cleaner suspensions than preparations from nontreated animals. Most of the treponemes in the purified suspensions were still motile, and none carried demonstrable host immunoglobulin. The preparations were free of recognizable host tissue debris and they contained, on the average, 1.9 × 10
−7
μg of protein per treponeme.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference11 articles.
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5. Specific agglutination of Treponema pallidum by sera from rabbits and human beings with treponemal infections;Hardy P. H.;J. Exp. Med.,1955
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