Affiliation:
1. Department of Biochemistry, University of California, Riverside, California 92521
Abstract
Acetylene generated from various grades of calcium carbide and obtained from commercial- and purified-grade acetylene cylinders was shown to contain high concentrations of various contaminants. Dependent on the source of acetylene, these included, at maximal values, H
2
(0.023%), O
2
(0.779%), N
2
(3.78%), PH
3
(0.06%), CH
4
(0.073%), and acetone (1 to 10%). The concentration of the contaminants in cylinder acetylene was highly dependent on the extent of cylinder discharge. Several conventional methods used to partially purify cylinder acetylene were compared. A small-scale method for extensively purifying acetylene is described. An effect of acetylene quality on acetylene reduction assays conducted with purified nitrogenase from
Azotobacter vinelandii
was demonstrated.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference24 articles.
1. Arp D. J. 1985. H2-oxidizing hydrogenases of aerobic N2-fixing microorganisms p. 121-131. In P. W. Ludden and J. E. Burris (ed.) Nitrogen fixation and CO2 metabolism Proceedings of the Fourteenth Steenbock Symposium. Elsevier Science Publishing Co. Inc. New York.
2. Kinetic mechanism of the hydrogen-oxidizing hydrogenase from soybean nodule bacteroids;Arp D. J.;Biochemistry,1981
3. Burris R. H. 1974. Methodology p. 23. In A. Quispel (ed.) The biology of nitrogen fixation. North Holland Publishing Co. Amsterdam.
4. Metabolism of acetylene by Rhodococcus Al;deBont J. A. M.;Arch. Microbiol.,1980
5. Nitrogenase-hydrogenase relationships in Rhizobium japonicum;Graham L. A.;Arch. Microbiol.,1984
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