Multilaboratory Assessment of Epstein-Barr Virus Serologic Assays: the Case for Standardization

Author:

Liu Zhiwei1ORCID,Yu Kelly J.1,Coghill Anna E.12,Brenner Nicole3,Cao Su-Mei4,Chen Chien-Jen5,Chen Yufeng6,Doolan Denise L.7,Hsu Wan-Lun5,Labo Nazzarena8,Middeldorp Jaap M.9,Miley Wendell8,Simon Julia310,Wang Cheng-Ping11,Waterboer Tim3,Whitby Denise8,Xie Shang-Hang4,Ye Weimin6,Hildesheim Allan1

Affiliation:

1. Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland, USA

2. Cancer Epidemiology Program, Division of Population Sciences, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida, USA

3. Infections and Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany

4. Department of Cancer Prevention Research, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China

5. Genomics Research Center, Academia Sinica, Taipei, Taiwan

6. Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden

7. Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia

8. Viral Oncology Section, AIDS and Cancer Virus Program, Leidos-Biomedical, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA

9. Department of Pathology, VU University Medical Center, Amsterdam, Netherlands

10. Faculty of Biosciences, Heidelberg University, Heidelberg, Germany

11. Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan

Abstract

IgA antibodies targeting Epstein-Barr virus (EBV) have been proposed for screening for nasopharyngeal carcinoma (NPC). However, methods differ, and the antigens used in these assays differ considerably between laboratories. To enable formal comparisons across a range of established EBV serology assays, we created a panel of 66 pooled serum samples and 66 pooled plasma samples generated from individuals with a broad range of IgA antibody levels.

Funder

HHS | NIH | National Cancer Institute

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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