Purification of a Streptococcal Bacteriocin (Viridin B) and Its Separation from Alpha-Hemolysin-Reference-Cited by-同舟云学术

Purification of a Streptococcal Bacteriocin (Viridin B) and Its Separation from Alpha-Hemolysin

Published:1979-03 Issue:3 Volume:15 Page:436-439
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Apelgren Lynn D.¹,Dajani Adnan S.¹

Affiliation:

1. Division of Infectious Diseases, Children's Hospital of Michigan and Department of Pediatrics, Wayne State University School of Medicine, Detroit, Michigan 48201

Abstract

Viridin B, a bacteriocin produced by Streptococcus mitis , copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.15.3.436

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