Preparation of Purified Suspensions of Coxiella burneti by Genetron Extraction Followed by Continuous-Flow Ultracentrifugation-Reference-Cited by-同舟云学术

Preparation of Purified Suspensions of Coxiella burneti by Genetron Extraction Followed by Continuous-Flow Ultracentrifugation

Published:1972-05 Issue:5 Volume:23 Page:841-845
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Dubois Doria R.¹²,Cutchins Ernest C.¹²,Berman Sanford¹²,Lowenthal Joseph P.¹²,Timchak Robert L.¹²

Affiliation:

1. Department of Biologics Research, Walter Reed Army Institute of Research, Washington, D.C. 20012

2. Department of Biology, Catholic University, Washington, D.C. 20017

Abstract

A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.23.5.841-845.1972

Reference19 articles.

1. Preparation of cholera vaccines by fluorocarbon extraction of cholera vibrio suspensions;Altieri P. L.;Infect. Immunity,1970

2. Continuous-flow centrifugation combined with isopycnic banding: rotors B-8 and B-IX;Anderson N. G.;Nat. Cancer Inst. Monogr.,1966

3. Method for the production of a purified dry Q fever vaccine;Berman S.;J. Bacteriol.,1961

4. The phase antigens of Coxiella bumeti;Bobb D.;Can. J. Microbiol.,1962

5. Improved continuous-flow centrifugation with banding;Cline G. B.;Nature (London),1966

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