Variability of the Influence of Physicochemical Factors Affecting Bacterial Adhesion to Polystyrene Substrata

Author:

McEldowney Sharron1,Fletcher Madilyn1

Affiliation:

1. Department of Environmental Sciences, Warwick University, Coventry CV4 7AL, and River Laboratory, Freshwater Biological Association, Wareham, Dorset BH2D 6BB, 2 England

Abstract

The role of electrostatic and hydrophobic interactions and solid and liquid surface tensions in the adhesion of four bacterial species ( Pseudomonas fluorescens, Enterobacter cloacae, Chromobacterium sp., and Flexibacter sp.) to hydrophobic polystyrene petri dishes and to more hydrophilic polystyrene tissue culture dishes was investigated. The effect of electrostatic interactions was investigated by determining the effects of different electrolyte solutions on attachment to and of different electrolyte and pH solutions on detachment from the polystyrene substrate. The significance of solid and liquid surface tensions and hydrophobic interactions was investigated by measuring the effects of different surfactants (including a concentration series of dimethyl sulfoxide) on adhesion and detachment. Adhesion varied with bacterial species, substratum, and electrolyte type and concentration, with no apparent correlation between adhesion and electrolyte valence or concentration. The influence of different pH and detergent solutions on bacterial detachment also varied with species, substratum, pH, and detergent type; however, the greatest degree of detachment of all strains from the surfaces was produced by detergent treatment. The results suggest that adhesion cannot be attributed to any one type of adhesive interaction. There was some evidence for both electrostatic and hydrophobic interactions, but neither interaction could wholly account for the data.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference35 articles.

1. The influence of ionic strength, pH and protein layer on the interaction between Streptococcus mutans and glass surfaces;Abbott A.;J. Gen. Microbiol.,1983

2. Surface thermodynamics of bacterial adhesion;Absolom D. R.;Appl. Environ. Microbiol.,1983

3. Interfacial phenomena and biomaterials;Andrade J. D.;Med. Instrum.,1973

4. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion;Busscher H. J.;Appl. Environ. Microbiol.,1984

5. Adhesion: a tactic in the survival strategy of a marine vibrio during starvation;Dawson M. P.;Curr. Microbiol.,1981

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3