Affiliation:
1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111
Abstract
ABSTRACT
The
Bacillus subtilis rocG
gene, encoding catabolic glutamate dehydrogenase, was found to be subject to direct CcpA-dependent glucose repression. The effect of CcpA required the presence of both the HPr and Crh proteins. The primary CcpA binding site was identified by mutational analysis and DNase I footprinting. In the absence of inducers of the Roc pathway,
rocG
was still expressed at a low level due to readthrough transcription. CcpA-dependent repression of
rocG
readthrough transcription proved to contribute to the slow growth rate of
B. subtilis
cells in glucose-glutamate medium. Increased readthrough expression of
rocG
was shown to be partially responsible for the growth defect of
ccpA
strains in glucose-ammonium medium.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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