Nitrosomonas europaea Expresses a Nitric Oxide Reductase during Nitrification

Author:

Beaumont Hubertus J. E.1,van Schooten Bas1,Lens Sylvia I.1,Westerhoff Hans V.1,van Spanning Rob J. M.1

Affiliation:

1. BioCentrum Amsterdam, Department of Molecular Cell Physiology, Vrije Universiteit, NL-1081 HV Amsterdam, The Netherlands

Abstract

ABSTRACT In this paper, we report the identification of a norCBQD gene cluster that encodes a functional nitric oxide reductase (Nor) in Nitrosomonas europaea . Disruption of the norB gene resulted in a strongly diminished nitric oxide (NO) consumption by cells and membrane protein fractions, which was restored by the introduction of an intact norCBQD gene cluster in trans . NorB-deficient cells produced amounts of nitrous oxide (N 2 O) equal to that of wild-type cells. NorCB-dependent activity was present during aerobic growth and was not affected by the inactivation of the putative fnr gene. The findings demonstrate the presence of an alternative site of N 2 O production in N. europaea .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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