Nitrite Reductase of Nitrosomonas europaea Is Not Essential for Production of Gaseous Nitrogen Oxides and Confers Tolerance to Nitrite-Reference-Cited by-同舟云学术

Nitrite Reductase of Nitrosomonas europaea Is Not Essential for Production of Gaseous Nitrogen Oxides and Confers Tolerance to Nitrite

Published:2002-05 Issue:9 Volume:184 Page:2557-2560
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Beaumont Hubertus J. E.¹,Hommes Norman G.²,Sayavedra-Soto Luis A.²,Arp Daniel J.²,Arciero David M.³,Hooper Alan B.³,Westerhoff Hans V.¹,van Spanning Rob J. M.¹

Affiliation:

1. BioCentrum Amsterdam, Department of Molecular Cell Physiology, Vrije Universiteit, NL-1081 Amsterdam, The Netherlands

2. Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331-2902

3. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, Minnesota 55108

Abstract

ABSTRACT A gene that encodes a periplasmic copper-type nitrite reductase (NirK) was identified in Nitrosomonas europaea . Disruption of this gene resulted in the disappearance of Nir activity in cell extracts. The nitrite tolerance of NirK-deficient cells was lower than that of wild-type cells. Unexpectedly, NirK-deficient cells still produced nitric oxide (NO) and nitrous oxide (N 2 O), the latter in greater amounts than that of wild-type cells. This demonstrates that NirK is not essential for the production of NO and N 2 O by N. europaea . Inactivation of the putative fnr gene showed that Fnr is not essential for the expression of nirK .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.184.9.2557-2560.2002

Reference25 articles.

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