Validation of a Self-Excising Marker in the Human Pathogen Aspergillus fumigatus by Employing the β-Rec/ six Site-Specific Recombination System-Reference-Cited by-同舟云学术

Validation of a Self-Excising Marker in the Human Pathogen Aspergillus fumigatus by Employing the β-Rec/ six Site-Specific Recombination System

Published:2010-09-15 Issue:18 Volume:76 Page:6313-6317
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hartmann Thomas¹,Dümig Michaela¹,Jaber Basem M.²,Szewczyk Edyta¹,Olbermann Patrick¹,Morschhäuser Joachim³,Krappmann Sven¹

Affiliation:

1. Research Center for Infectious Diseases

2. Department of Biological Sciences, University of Jordan, Amman, Jordan

3. Institute for Molecular Infection Biology, Julius-Maximilians-University Würzburg, Würzburg, Germany

Abstract

ABSTRACT Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing β serine recombinase acting on six recognition sequences (β-rec/ six ) in a fungal host, the human pathogen Aspergillus fumigatus , and its use in establishing a self-excising resistance marker cassette for serial gene replacement.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00882-10

Reference46 articles.

1. Alonso, J. C., C. Gutierrez, and F. Rojo. 1995. The role of chromatin-associated protein Hbsu in beta-mediated DNA recombination is to facilitate the joining of distant recombination sites. Mol. Microbiol.18:471-478.

2. Alonso, J. C., F. Weise, and F. Rojo. 1995. The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the β recombinase of plasmid pSM19035. J. Biol. Chem.270:2938-2945.

3. Bayram, Ö., S. Krappmann, M. Ni, J. W. Bok, K. Helmstaedt, O. Valerius, S. Braus-Stromeyer, N. J. Kwon, N. P. Keller, J. H. Yu, and G. H. Braus. 2008. VelB/VeA/LaeA complex coordinates light signal with fungal development and secondary metabolism. Science320:1504-1506.

4. Canosa, I., F. Rojo, and J. C. Alonso. 1996. Site-specific recombination by the β protein from the streptococcal plasmid pSM19035: minimal recombination sequences and crossing over site. Nucleic Acids Res.24:2712-2717.

5. The akuB KU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus

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