The akuB KU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus-Reference-Cited by-同舟云学术

The akuB KU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus

Published:2006-01 Issue:1 Volume:5 Page:207-211
ISSN:1535-9778
Container-title:Eukaryotic Cell
language:en
Short-container-title:Eukaryot Cell

Author:

da Silva Ferreira Márcia Eliana¹,Kress Marcia R. V. Z.¹,Savoldi Marcela¹,Goldman Maria Helena S.²,Härtl Albert³,Heinekamp Thorsten⁴,Brakhage Axel A.⁴,Goldman Gustavo H.¹

Affiliation:

1. Faculdade de Ciências Farmacêuticas de Ribeirão Preto

2. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil

3. Drug Testing Group

4. Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knoell-Institut, Jena, Germany

Abstract

ABSTRACT To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB KU80 ). Homologous integration reached about 80% for both calcineurin A ( calA ) and polyketide synthase pksP (alb1 ) genes in the akuB KU80 mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB KU80 had no influence on pathogenicity in a low-dose murine infection model.

Publisher

American Society for Microbiology

Subject

Molecular Biology,General Medicine,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/EC.5.1.207-211.2006

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