Multicenter Evaluation of Two Next-Generation HIV-1 Quantitation Assays, Aptima Quant Dx and Cobas 6800, in Comparison to the RealTi m e HIV-1 Reference Assay-Reference-Cited by-同舟云学术

Multicenter Evaluation of Two Next-Generation HIV-1 Quantitation Assays, Aptima Quant Dx and Cobas 6800, in Comparison to the RealTi m e HIV-1 Reference Assay

Published:2018-10 Issue:10 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wiesmann Frank¹,Ehret Robert²,Naeth Gudrun¹,Däumer Martin³,Fuhrmann Jörg⁴,Kaiser Rolf⁵,Noah Christian⁶,Obermeier Martin²,Schalasta Gunnar⁷,Tiemann Carsten⁸,Wolf Eva⁹,Knechten Heribert¹,Braun Patrick¹

Affiliation:

1. PZB Aachen, Medical Center for Infectious Diseases, Aachen, Germany

2. MVZmib AG, Medical Center for Infectious Diseases, Berlin, Germany

3. IIG-Institute of Immunology and Genetics, Kaiserslautern, Germany

4. Labor 28, Berlin, Germany

5. University of Cologne, Institute of Virology, Cologne, Germany

6. MVZ Labor Lademannbogen, Hamburg, Germany

7. Labor Enders, Laboratory for Medical Diagnostics, Stuttgart, Germany

8. Labor Krone, Laboratory for Medical Diagnostics, Bad Salzuflen, Germany

9. MUC Research, Munich, Germany

Abstract

High accuracy and precision at the lower end of quantification are crucial requirements of a modern HIV viral load (VL) assay, since some clinically relevant thresholds are located at 50 and 200 copies/ml. In this study, we compared the performance of two new fully automated HIV-1 VL assays, Aptima HIV-1 Quant Dx and Cobas HIV-1 (Cobas 6800), with the established RealTi m e m 2000 assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00292-18

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