Nucleotide sequence and intracellular location of the product of the fosfomycin resistance gene from transposon Tn2921

Author:

Navas J1,León J1,Arroyo M1,García Lobo J M1

Affiliation:

1. Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.

Abstract

An 863-base-pair DNA fragment containing the fosfomycin resistance gene from transposon Tn2921 was sequenced. Analysis of the sequence revealed the presence of a single open reading frame that encoded the FOS polypeptide of 16 kilodaltons from Tn2921. Minicell fractionation studies showed that the FOS protein was located in the bacterial cytoplasm.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference17 articles.

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3. Davis R. W. D. Botstein and J. Roth. 1980. Advanced bacterial genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

4. The transposon Tn) as a probe for studying ColEl structure and function;Dougan G.;Mol. Gen. Genet.,1977

5. Cloning and expression in minicells of the determinant of resistance to fosfomycin from the transposon Tn 2921;Garcia-Lobo J. M.;Plasmid,1984

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