Identification and Characterization of a Novel FosA7 Member from Fosfomycin-Resistant Escherichia coli Clinical Isolates from Canadian Hospitals-Reference-Cited by-同舟云学术

Identification and Characterization of a Novel FosA7 Member from Fosfomycin-Resistant Escherichia coli Clinical Isolates from Canadian Hospitals

Published:2020-12-16 Issue:1 Volume:65 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Milner Kieran A.¹,Bay Denice C.¹^ORCID,Alexander David¹²,Walkty Andrew¹³^ORCID,Karlowsky James A.¹³,Mulvey Michael R.¹⁴,Sharma Meenu K.¹⁴,Zhanel George G.¹

Affiliation:

1. Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada

2. Cadham Provincial Laboratory, Winnipeg, Canada

3. Shared Health, Winnipeg, Canada

4. Public Health Agency of Canada–National Microbiology Laboratory, Winnipeg, Canada

Abstract

Here, we characterize the fosA genes from three Escherichia coli clinical isolates recovered from Canadian patients. Each fosA sequence was individually overexpressed in E. coli BW25113, and antimicrobial susceptibility testing was performed to assess their role in fosfomycin resistance. The findings from this study identify and functionally characterize FosA3, FosA8, and novel FosA7 members and highlight the importance of phenotypic characterization of fosA genes.

Funder

Shared Health

University of Manitoba

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00865-20

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